Dr Noburo Sato's lab has found that the use of blebbistatin, a non-muscle myosin II (NMII) inhibitor, enhances cloning efficiency of human ES and iPS cells. The effect of blebbistatin is comparable to that of ROCK inhibitor, Y-27632 in both adherent monolayer and suspension culture conditions. shRNA-mediated knockdown of NMII heavy chain (NMHCIIA) also prevented...